1011c: DESULFOTHERMUS MJ MEDIUM (H2/CO2)
Modifications: Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5.
Growth observed:
Growth conditions: 2-3 days anaerobic 37 °C